Clathrin Heavy Chain 1/CHC17 Antibody (CLTC/1431)

• Reactivity: Hu
• Applications: ELISA
• Clone: CLTC/1431
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Clathrin Heavy Chain 1/CHC17 Antibody (CLTC/1431) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54300)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Purified recombinant N-terminal fragment of human Clathrin Heavy Chain 1/CHC17
• Localization: Cytoplasmic
• Specificity: Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLTC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: ELISA: For coating, order Ab without BSA.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (CLTC/1431)

• CHC
• CHC17
• Clathrin Heavy Chain 1
• Clathrin heavy chain on chromosome 17
• clathrin, heavy chain (Hc)
• clathrin, heavy chain
• clathrin, heavy polypeptide (Hc)
• clathrin, heavy polypeptide-like 2
• CLH17
• CLH-17
• CLTC
• CLTCL2
• Hc
• KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLTC
• Uniprot:
  • P53675()
  • P53675()
  • Q00610(Human)