| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-613-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49293-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99308-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6784-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99436-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08333 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22654-50ul | |||
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Western-Blot-NB300-613-img0014.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in Hela Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0012.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Normal biopsies of deparaffinized Human colon tissue.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunohistochemistry-Paraffin-NB300-613-img0013.jpg)
![Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Alexa Fluor (R) 488 conjugate of NB300-613. A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an a](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Flow-Cytometry-NB300-613-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in U251 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0011.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunohistochemistry-Paraffin-NB300-613-img0007.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] - Analysis of extracts of various cell lines, using Clathrin Heavy Chain 1/CHC17 Rabbit mAb (NBP3-16518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-9M2G2-Western-Blot-NBP3-16518-img0005.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] - Analysis of extracts of various cell lines, using Clathrin Heavy Chain 1/CHC17 Rabbit mAb (NBP3-16518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug Clathrin Heavy Chain 1/CHC17 antibody (NBP3-16518). Western blot was performed from the immunoprecipitate using Clathrin Heavy Chain 1/CHC17 antibody (NBP3-16518) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-9M2G2-Immunoprecipitation-NBP3-16518-img0006.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] -Human colon carcinoma tissue using Clathrin heavy chain Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16518_rabbit-clathrin-heavy-chain-1-chc17-mab-9m2g2-immunohistochemistry-paraffin-412202411547..png)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] - Human colon tissue using Clathrin heavy chain Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16518_rabbit-clathrin-heavy-chain-1-chc17-mab-9m2g2-immunohistochemistry-paraffin-412202411654..png)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [NBP3-16518] -Human thyroid cancer tissue using Clathrin heavy chain Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16518_rabbit-clathrin-heavy-chain-1-chc17-mab-9m2g2-immunohistochemistry-paraffin-412202411813..png)
![Immunocytochemistry/ Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (9M2G2) [Clathrin Heavy Chain 1/CHC17] - Confocal imaging of HeLa cells using Clathrin Heavy Chain 1/CHC17 Rabbit mAb . The cells were counterstained with alpha-Tubulin Rabbit mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-16518_rabbit-clathrin-heavy-chain-1-chc17-mab-9m2g2-5220251735274.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human cell line U-251 MG shows localization to endosomes & lysosomes.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49293-img0004.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human cell line U-251 MG shows localization to endosomes & lysosomes.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-49293-img0008.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-49293-img0005.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human placenta shows strong membranous positivity in trophoblstic cells.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-49293-img0006.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-49293-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] - SHSY5Y cells differentiated into neurons. Green: CLTC antibody diluted 1:100 in PBS-Triton 0.2%X-100/BSA 1%. 2 hours at RT. Blue: DAPI. Image from verified customer review](http://images.novusbio.com/fullsize/antibody/nbp2-49293_rabbit-polyclonal-clathrin-heavy-chain-1-chc17-antibody-162023151622.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-49293] -Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/antibody/nbp2-49293_rabbit-polyclonal-clathrin-heavy-chain-1-chc17-antibody-western-blot-301202511558..png)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 191 kDa](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Western-Blot-NBP2-99308-img0007.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 191 kDa")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0006.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0002.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0003.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0004.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99308] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99308-img0005.jpg)
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Western-Blot-NBP2-99436-img0006.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Anti-Clathrin Heavy Chain 1/CHC17 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse brain tissue Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 180 kDa. Observed band size: 180 kDa")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in rat kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0005.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0002.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0003.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP2-99436] - Immunochemical staining Clathrin Heavy Chain 1/CHC17 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP2-99436-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-BF-06-Immunofluorescence-NBP1-45050-img0002.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-BF-06-Immunofluorescence-NBP1-45050-img0001.jpg)
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP1-52529] - Analysis of anti-CLTC antibody with human adrenal at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP1-52529-img0002.jpg "Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP1-52529] - Analysis of anti-CLTC antibody with human adrenal at concentration 3.75 ug/ml.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody [NBP1-52529] - Analysis of anti-CLTC antibody with human kidney at concentration 3.75 ug/ml.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-Immunohistochemistry-Paraffin-NBP1-52529-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432) [NBP3-07569] - Confocal Immunofluorescence image of HeLa cells stained with Clathrin Heavy Chain 1/CHC17Monoclonal Antibody (CHC/1432) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuceli red.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-CHC-1432-Immunocytochemistry-Immunofluorescence-NBP3-07569-img0001.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (CHC/1432) [NBP3-07569] - Confocal Immunofluorescence image of HeLa cells stained with Clathrin Heavy Chain 1/CHC17Monoclonal Antibody (CHC/1432) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuceli red.")
![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). Predicted band size: 192kDa. Observed band size: 192kDa.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-S02-6C4-Western-Blot-NBP3-19848-img0002.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Western blot detection of Clathrin Heavy Chain 1/CHC17 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19848 (1:1000 diluted). Predicted band size: 192kDa. Observed band size: 192kDa.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (S02-6C4) [NBP3-19848] - Immunohistochemistry of Clathrin Heavy Chain 1/CHC17 in paraffin-embedded Human colon cancer tissue using NBP3-19848 at dilution 1/50](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-S02-6C4-Immunohistochemistry-Paraffin-NBP3-19848-img0001.jpg)
![Western Blot: Clathrin Heavy Chain Antibody (SR1305) [NBP3-22654] - Western blot analysis using NBP3-22654 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22654_rabbit-clathrin-heavy-chain-mab-sr1305-5720231624744.jpg "Western Blot: Clathrin Heavy Chain Antibody (SR1305) [NBP3-22654] - Western blot analysis using NBP3-22654 in Hela cell lysate.")
![Immunoprecipitation:[NBP3-30732]-Detection of human CHC by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody used for IP at 6 µg per reaction. CHC was also immunoprecipitated by rabbit anti-CHC antibody. For blotting immunoprecipitated CHC, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30732_rabbit-clathrin-heavy-chain-1-chc17-pab-2312025128856.jpg "Immunoprecipitation:[NBP3-30732]-Detection of human CHC by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHC antibody used for IP at 6 µg per reaction. CHC was also immunoprecipitated by rabbit anti-CHC antibody. For blotting immunoprecipitated CHC, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")