Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) - BSA Free

Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: BF-06
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) - BSA Free Summary

• Immunogen: The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
• Marker: Clathrin-coated Vesicle Marker
• Specificity: The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLTC
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Stabilizing Tris buffered saline (TBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Multi-step

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) - BSA Free

• CHC
• CHC17
• Clathrin Heavy Chain 1
• Clathrin heavy chain on chromosome 17
• clathrin, heavy chain (Hc)
• clathrin, heavy chain
• clathrin, heavy polypeptide (Hc)
• clathrin, heavy polypeptide-like 2
• CLH17
• CLH-17
• CLTC
• CLTCL2
• Hc
• KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLTC