Clathrin Heavy Chain 1/CHC17 Antibody (BF-06)

Images

 
Immunocytochemistry/ Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by ...read more
Immunocytochemistry/ Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) [NBP1-45050] - Staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP
Clone
BF-06
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Clathrin Heavy Chain 1/CHC17 Antibody (BF-06) Summary

Immunogen
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
Marker
Clathrin-coated Vesicle Marker
Specificity
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CLTC
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Porcine, Mouse, Rat and Bovine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (approx. pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (BF-06)

  • CHC
  • CHC17
  • Clathrin Heavy Chain 1
  • Clathrin heavy chain on chromosome 17
  • clathrin, heavy chain (Hc)
  • clathrin, heavy chain
  • clathrin, heavy polypeptide (Hc)
  • clathrin, heavy polypeptide-like 2
  • CLH17
  • CLH-17
  • CLTC
  • CLTCL2
  • Hc
  • KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45050AF405
Alexa Fluor 488 Labeled NBP1-45050AF488
Alexa Fluor 647 Labeled NBP1-45050AF647
Alexa Fluor 700 Labeled NBP1-45050AF700
Allophycocyanin Labeled NBP1-45050APC
DyLight 350 Labeled NBP1-45050UV
DyLight 405 Labeled NBP1-45050V
DyLight 488 Labeled NBP1-45050G
DyLight 680 Labeled NBP1-45050FR
DyLight 755 Labeled NBP1-45050IR
PE Labeled NBP1-45050PE
PerCP Labeled NBP1-45050PCP

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Diseases for Clathrin Heavy Chain 1/CHC17 Antibody (NBP1-45050)

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Bioinformatics

Gene Symbol CLTC
Entrez