CKAP4/p63 Antibody (1086526) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 1086526
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

CKAP4/p63 Antibody (1086526) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived human CKAP-4
  His128-Val602
  Accession # Q07065
• Specificity: Detects recombinant human CKAP4 in Direct ELISA.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CKAP4/p63 Antibody (1086526) [Alexa Fluor® 700]

• 63 kDa membrane protein
• CKAP4
• CLIMP-63
• cytoskeleton-associated protein 4
• ERGIC-63
• MGC99554
• p63
• transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment
• type-II transmembrane protein p63

Background

CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels). Human CKAP4 is a 602 amino acid (aa) type II transmembrane nonglycosylated protein. It contains a 106 aa N-terminal cytoplasmic region plus a 475 aa C-terminal luminal domain (aa 128-602). The luminal domain contains three coiled-coil regions (aa 130-214; 256-460; 533-602) plus three utilized phosphorylation sites. CKAP4 undergoes reversible palmitoylation. There are two potential isoform variants. One contains an alternative start at Met269, while another shows a deletion of aa 258-435. Over aa 128-602, human CKAP4 shares 82% aa sequence identity with mouse CKAP4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.