cIAP-1/HIAP-2 Antibody

Immunohistochemistry: cIAP-1/HIAP-2 Antibody - Unpurified [NB100-56130] - cIAP-1/HIAP-2 Antibody [NB100-56130] - Analysis of formalin-fixed, paraffin-embedded human breast tissue using this antibody at 1:2000. A, normal breast tissue. B, ductal carcinoma in situ (DCIS). C, invasive breast carcinoma. A1, B1, and C1 are higher magnifications of A, B and C, respectively. Hematoxylin-eosin counterstain. Increased cIAP1 expression is seen in the cancerous compared to normal breast tissue. Hematoxylin-eosin counterstain.

Immunohistochemistry: cIAP-1/HIAP-2 Antibody - Unpurified [NB100-56130] - cIAP-1/HIAP-2 Antibody [NB100-56130] - Analysis of a formalin-fixed, paraffin-embedded human ovarian cancer tissue microarray using this antibody at 1:2000. Differential expression of cIAP-1/HIAP-2 is seen between patient samples. Hematoxylin-eosin counterstain.

Product Discontinued

cIAP-1/HIAP-2 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 2-18 (HKTASQRLFPGPSYQNI) of human cIAP1 was used as immunogen, GenBank no. gi|2497238.
• Specificity: cIAP1 has 618 amino acids and migrates at ~70 kDa on SDS-PAGE. Observed molecular weights may vary as genes generally produce, by alternative splicing, multiple transcripts all with introns, putatively encoding multiple different protein isoforms. Additionally, proteins may undergo post translational modifications including phosphorylation, glycosylation and cleavage which can result in differential molecular weights. Protein modifications can vary between cell and tissue type, and proteins can migrate as one or multiple bands depending on the isoform expressed as well as post-translational modifications.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for cIAP-1/HIAP-2 Antibody

• API1Hiap-2
• apoptosis inhibitor 1
• baculoviral IAP repeat containing 2
• baculoviral IAP repeat-containing 2
• baculoviral IAP repeat-containing protein 2
• BIRC2
• cIAP1
• c-IAP1
• cIAP-1
• hIAP2
• HIAP-2
• IAP homolog B
• IAP2
• IAP-2
• Inhibitor of apoptosis protein 2
• MIHB
• MIHBHIAP2
• NFR2-TRAF signalling complex protein
• RING finger protein 48
• RNF48hiap-2
• TNFR2-TRAF-signaling complex protein 2

Background

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubiquitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIRC2
• Entrez:
  • 329(Human)