cIAP-1/HIAP-2 Antibody

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Immunohistochemistry: cIAP-1/HIAP-2 Antibody [NB100-56130] - Analysis of formalin-fixed, paraffin-embedded human breast tissue using NB100-56130 at 1:2000. A, normal breast tissue. B, ductal carcinoma in situ (DCIS). C, ...read more
Immunohistochemistry: cIAP-1/HIAP-2 Antibody [NB100-56130] - Analysis of a formalin-fixed, paraffin-embedded human ovarian cancer tissue microarray using NB100-56130 at 1:2000. Differential expression of cIAP-1/HIAP-2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

cIAP-1/HIAP-2 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 2-18 (HKTASQRLFPGPSYQNI) of human cIAP1 was used as immunogen, GenBank no. gi|2497238.
Specificity
cIAP1 has 618 amino acids and migrates at ~70 kDa on SDS-PAGE. Observed molecular weights may vary as genes generally produce, by alternative splicing, multiple transcripts all with introns, putatively encoding multiple different protein isoforms. Additionally, proteins may undergo post translational modifications including phosphorylation, glycosylation and cleavage which can result in differential molecular weights. Protein modifications can vary between cell and tissue type, and proteins can migrate as one or multiple bands depending on the isoform expressed as well as post-translational modifications.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BIRC2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for cIAP-1/HIAP-2 Antibody

  • API1Hiap-2
  • apoptosis inhibitor 1
  • baculoviral IAP repeat containing 2
  • baculoviral IAP repeat-containing 2
  • baculoviral IAP repeat-containing protein 2
  • BIRC2
  • cIAP1
  • c-IAP1
  • cIAP-1
  • hIAP2
  • HIAP-2
  • IAP homolog B
  • IAP2
  • IAP-2
  • Inhibitor of apoptosis protein 2
  • MIHB
  • MIHBHIAP2
  • NFR2-TRAF signalling complex protein
  • RING finger protein 48
  • RNF48hiap-2
  • TNFR2-TRAF-signaling complex protein 2

Background

cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on cIAP-1/HIAP-2.

cIAP1 - An apoptotic regulator with implications in drug resistant cancers
Cellular inhibitor of apoptosis protein-1 (cIAP-1) is an anti-apoptotic protein that is able to bind to caspases and inhibit their activity. Additionally cIAP-1 contains a RING domain with E3 ubiquitin ligase activity that is able to mediate the r...  Read full blog post.

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Bioinformatics

Gene Symbol BIRC2
Entrez