Chromogranin A Antibody (948107) [Unconjugated]

Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in chief cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 948107
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Chromogranin A Antibody (948107) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived human Chromogranin A
  Met1-Gly457
  Accession # P10645
• Specificity: Detects human Chromogranin A in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHGA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chromogranin A Antibody (948107) [Unconjugated]

• betagranin (N-terminal fragment of chromogranin A)
• Betagranin
• Catestatin
• CGA
• CHGA
• Chromofungin
• chromogranin A (parathyroid secretory protein 1)
• Chromogranin A
• chromogranin-A
• Pancreastatin
• Parastatin
• Parathyroid Secretory Protein 1
• Pituitary Secretory Protein I
• SP-I

Background

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Chromogranin A Antibody (MAB9098). (Showing 1 - 1 of 1 FAQ).

1. I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
   • When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: CHGA
• Uniprot:
  • P10645()