Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

Western Blot: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: hCG alpha (0.05ug) [+]. Lane 3: hCG alpha/U-20S WCL (0.05ug/20ug) [+]. Lane 4: U-20S WCL (20ug) [-]. Lane 5: HeLa WCL (35ug) [+]. Primary Antibody: Anti-alpha-hCG at 1:1000 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG mx8 HRP Conjugated at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 30 sec. Predicted MW: alpha subunit ~14.9kDa, full hCG ~37kDa, luteinizing hormone (LH) ~29kDa, thyroid stimulating hormone (TSH) ~28kDa, follicle stimulating hormone (FSH) ~30kDa. Observed MW: alpha subunit ~15-20kDa, full hCG ~35kDa.

ELISA: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody [NBP3-18630] - Each well was coated in duplicate at 10ug/mL of hCG alpha (RED) and full hCG (BLUE) protein. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking Buffer, Donkey Anti-Goat IgG MX8 HRP Conjugated Secondary, and TMB ELISA Peroxidase substrate.

• Reactivity: Hu
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free Summary

• Description: Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was affinity purified from monospecific antiserum by immunoaffinity purification.
• Immunogen: Prepared from whole goat serum produced by repeated immunizations with Human Chorionic Gonadotropin alpha Chain (hCG alpha) native protein.
• Specificity: This product reacts with Full Human Chorionic Gonadotropin (hCG) and Human Chorionic Gonadotropin Alpha chain (alpha-hCG).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CGA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot
• Application Notes: This product has been tested in ELISA and WB. Expect a band at ~14.9kDa for the alpha chain in western blot using appropriate tissues and lysates. ELISA reactivity expected against alpha hCG with an expected titer of ~1:100,000 and full hCG at ~1:50,000 dilution.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody - BSA Free

• alpha HCG
• CGA glycoprotein hormones, alpha polypeptide
• CGA
• CG-ALPHA
• Chorionic Gonadotropin alpha Chain
• FSHA
• GPHa
• GPHa1
• HCG
• LHA
• TSHA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CGA
• Uniprot:
  • P01215()
  • P01215()
  • P01215()
  • P01215()