Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) [NBP3-33064] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-Chorionic Gonadotropin alpha Chain (hCG alpha) antibody (NBP3-33064) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33064) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: AH21-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within full length human Chorionic Gonadotropin alpha Chain (hCG alpha). (Uniprot: P01215)
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

• alpha HCG
• CGA glycoprotein hormones, alpha polypeptide
• CGA
• CG-ALPHA
• Chorionic Gonadotropin alpha Chain
• FSHA
• GPHa
• GPHa1
• HCG
• LHA
• TSHA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CGA