Chk2 Antibody (1C12B8)


Western Blot: Chk2 Antibody (1C12B8) [NBP1-47549] - Analysis using CHK2 mouse mAb against cell lysate from various cell types.
Immunocytochemistry/ Immunofluorescence: Chk2 Antibody (1C12B8) [NBP1-47549] - Analysis of Hela cells using CHK2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 more
Immunohistochemistry-Paraffin: Chk2 Antibody (1C12B8) [NBP1-47549] - Analysis of human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Chk2 Antibody (1C12B8) Summary

Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide


  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence, ELISA.
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Chk2 Antibody (1C12B8)

  • CDS1
  • CHEK2
  • CHK2 checkpoint homolog (S. pombe)
  • EC 2.7.11
  • EC
  • HuCds1
  • LFS2
  • PP1425
  • Rad53
  • S.pombe) homolog


CHK2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block. Defects in CHEK2 are associated with Li-Fraumeni syndrome (LFS); a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are also found in some patients with prostate cancer (CaP) or osteosarcoma (OSRC). Substantial amino acid variants exist in some cancer tissues. Numerous truncated splice variants exist for this protein.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Func, ICC/IF, IP
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: IP (-), WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IP
Species: Hu
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Chk2 Antibody (NBP1-47549) (0)

There are no publications for Chk2 Antibody (NBP1-47549).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Chk2 Antibody (NBP1-47549) (0)

There are no reviews for Chk2 Antibody (NBP1-47549). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Chk2 Antibody (NBP1-47549) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Chk2 Antibody (1C12B8) Products

Related Products by Gene

Bioinformatics Tool for Chk2 Antibody (NBP1-47549)

Discover related pathways, diseases and genes to Chk2 Antibody (NBP1-47549). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Chk2 Antibody (NBP1-47549)

Discover more about diseases related to Chk2 Antibody (NBP1-47549).

Pathways for Chk2 Antibody (NBP1-47549)

View related products by pathway.

PTMs for Chk2 Antibody (NBP1-47549)

Learn more about PTMs related to Chk2 Antibody (NBP1-47549).

Research Areas for Chk2 Antibody (NBP1-47549)

Find related products by research area.

Blogs on Chk2

There are no specific blogs for Chk2, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol CHEK2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-47549 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought