Chitinase 3-like 1 Antibody (2C10)

Images

 
Western Blot: Chitinase 3-like 1 Antibody (2C10) [NBP1-28610] - SW480 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L1 (1:500). Proteins were visualized using a ...read more
Western Blot: Chitinase 3-like 1 Antibody (2C10) [NBP1-28610] - Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CHI3L1 (1:250). Proteins were visualized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Chitinase 3-like 1 Antibody (2C10) Summary

Immunogen
Recombinant human CHI3L1 (22-383aa) purified from E. coli (NP_001267).
Specificity
anti-human CHI3L1 mAb, clone 2C10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CHI3L1 protein
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHI3L1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1500
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Chitinase 3-like 1 Antibody (2C10)

  • AW208766
  • Brp39
  • CHI3L1
  • Chitinase 3 like 1
  • Chitinase 3-like 1
  • gp39
  • HCgp39
  • YKL-40

Background

Chitinase 3-Like-1 (CHI3L1) is a recently discovered human glycoprotein which is related in amino acid sequence to the chitinase protein family, but has no chitinase activity. CHI3L1 is upregulated in a number of cancers and in non-neoplatic disease states characterized by chronic inflammation and tissue remodeling. Its pattern of expression is associated with pathogenic processes related to inflammation, extracellular tissue remodeling, fibrosis and solid carcinomas. CHI3L1 may play an important role in the capacity of cells to respond to and cope with changes in their environment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHI3L1
Entrez
Uniprot