CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

Sandwich ELISA: CHIP/STUB1 Antibody (2E12) [H00010273-M01] - Detection limit for recombinant GST tagged STUB1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CHIP/STUB1 Antibody (2E12) - Azide and BSA Free Summary

• Immunogen: STUB1 (NP_005852.2, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY
• Specificity: Reacts with STIP1 homology and U-box containing protein 1.
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: STUB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CHIP/STUB1 Antibody (2E12) - Azide and BSA Free

• Antigen NY-CO-7
• Carboxy terminus of Hsp70-interacting protein
• CHIP
• CHIPSTIP1 homology and U box-containing protein 1
• CLL-associated antigen KW-8
• E3 ubiquitin-protein ligase CHIP
• EC 6.3.2.-
• heat shock protein A binding protein 2 (c-terminal)
• HSPABP2
• NY-CO-7
• SDCCAG7
• serologically defined colon cancer antigen 7
• STIP1 homology and U-box containing protein 1
• STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
• STUB1
• UBOX1

Background

STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CHIP/STUB1 Antibody (H00010273-M01). (Showing 1 - 1 of 1 FAQ).

1. I am interested in a monoclonal antibody for Stub1 protein (CHIP). I need the antibody for a structural characterization, so it's very important for me to know the reactive sequence. It would be great if you can give me information about what is the reactive domain (TPR or U-box).
   • You are right to identify CHIP Antibody (2E12) (H00010273-M01) - this is our only monoclonal antibody for STUB1/CHIP. The immunogen sequence for H00010273-M01 is amino acids 204-303 of the canonical sequence (isoform 1) as described in UniProt (http://www.uniprot.org/uniprot/Q9UNE7). According to this UniProt page (the 'Regions' section), this corresponds to the U-box domain, with some amino acids either side, but not overlapping with a TPR repeat.

Bioinformatics

• Gene Symbol: STUB1
• Entrez:
  • 10273(Human)
• Uniprot:
  • NP_005852(Human)