CHFR Antibody (2B11)

Images

 
Western Blot: CHFR Antibody (2B11) [H00055743-M08] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M08), clone 2B11. Lane 1: CHFR transfected lysatE (72.1 KDa). Lane 2: ...read more
Sandwich ELISA: CHFR Antibody (2B11) [H00055743-M08] - Detection limit for recombinant GST tagged CHFR is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHFR Antibody (2B11) Summary

Immunogen
CHFR (AAH12072.1, 136 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSC
Specificity
CHFR - checkpoint with forkhead and ring finger domains (2B11)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHFR
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CHFR Antibody (2B11)

  • Checkpoint with forkhead and RING finger domains protein
  • checkpoint with forkhead and ring finger domains
  • E3 ubiquitin-protein ligase CHFR
  • EC 6.3.2.-
  • FLJ10796
  • FLJ33629
  • RING finger protein 196
  • RNF196RNF116

Background

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHFR
Entrez
Uniprot