ChemR23/CMKLR1 Antibody (84939) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PerCP-conjugated Monoclonal Antibody (FAB301C) and either (A) Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P) or (B) Mouse IgG3Phycoerythrin Isotype Control (IC007P). View our protocol for Staining Membrane-associated Proteins.

HEK293 human cell line (A) transfected with human ChemR23 or (B) irrelevant protein and eGFP, were stained with Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P). Quadrant markers were set based on Mouse IgG3 PE Isotype Control (IC007P, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 84939
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ChemR23/CMKLR1 Antibody (84939) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human ChemR23
  Met1-Leu371
  Accession # NP_004063
• Specificity: Detects human ChemR23. Stains human ChemR23-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: CMKLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ChemR23/CMKLR1 Antibody (84939) [Phycoerythrin]

• CHEMERINR
• chemokine receptor-like 1
• chemokine-like receptor 1
• ChemR23
• cmklr1
• Dez
• Gpcr27
• G-protein coupled receptor ChemR23
• G-protein coupled receptor DEZ
• MGC126105
• MGC126106
• orphan G-protein coupled receptor, Dez

Background

ChemR23 is a chemoattractant receptor expressed on dendritic cells and activated macrophages. ChemR23 binds Chemerin and functions as a coreceptor for SIV and some primary HIV-1 strains.

1. Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ChemR23/CMKLR1 Antibody (FAB362P)(2)

Publications using FAB362P

• Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A. Schneider, Medhanie A. Mulaw et al. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration Oncotarget 2016-12-13 [PMID: 27907906]
• Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A. Schneider, Medhanie A. Mulaw et al. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration Oncotarget 2016-12-13 [PMID: 27907906] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CMKLR1
• Uniprot:
  • NP_004063()