CHD5 Antibody

Western Blot: CHD5 Antibody [NBP3-18636] - Figure 2. Western blot analysis using the antibody directed against CHD5 HeLa cells were transfected with CHD5 and analysed by Western blot. Western blot was performed using 15 to 30 ug nuclear extracts and the antibody against human CHD5 . The antibody was diluted 1:1,000 in TBS-Tween containing 2% milk powder. The position of the protein of interest (expected size: 223 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells. Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.

ELISA: CHD5 Antibody [NBP3-18636] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human CHD5 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CHD5 Antibody Summary

• Description: Novus Biologicals Rabbit CHD5 Antibody (NBP3-18636) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human CHD5 (chromodomain helicase DNA binding protein 5), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the central part of the protein, respectively.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD5
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:500-1:1000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for CHD5 Antibody

• ATP-dependent helicase CHD5
• CHD-5
• chromodomain helicase DNA binding protein 5
• chromodomain-helicase-DNA-binding protein 5
• DKFZp434N231
• EC 3.6.1
• EC 3.6.4.12
• KIAA0444

Background

Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 (PubMed 12592387)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHD5
• Uniprot:
  • Q8TDI0(Human)