CHD1L Antibody - BSA Free

Western Blot: CHD1L Antibody [NBP2-59305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CHD1L Antibody [NBP2-59305] - CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 ug of CHD1L antibody 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody diluted at 1:1000.

Western Blot: CHD1L Antibody [NBP2-59305] - Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 5% SDS PAGE. Used at a dilution of 1:3000

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CHD1L Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CHD1L Antibody - BSA Free (NBP2-59305) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CHD1L. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD1L
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CHD1L Antibody - BSA Free

• ALC1
• amplified in liver cancer 1
• amplified in liver cancer protein 1
• CHD1L
• CHDL
• chromodomain helicase DNA binding protein 1-like
• chromodomain-helicase-DNA-binding protein 1-like
• FLJ22530

Background

In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 [PubMed 19661379]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CHD1L