ch-TOG Antibody - BSA Free

Western Blot: ch-TOG Antibody [NBP2-92555] - Analysis of extracts of various cell lines, using ch-TOG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of Mouse L929 cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of U2OS cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP2-92555] - Immunofluorescence analysis of Rat H9C2 cells using ch-TOG antibody (NBP2-92555) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation: ch-TOG Antibody [NBP2-92555] - Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug ch-TOG antibody (NBP2-92555). Western blot was performed from the immunoprecipitate using ch-TOG antibody (NBP2-92555) at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ch-TOG Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1). LLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKAP5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ch-TOG Antibody - BSA Free

• CHTOG
• Ch-TOG
• Colonic and hepatic tumor over-expressed gene protein
• cytoskeleton associated protein 5
• cytoskeleton-associated protein 5
• EC 2.7.7.6
• EC 3.1.1.29
• KIAA0097FLJ35359
• MSPS
• TOG
• TOGp

Background

ch-TOG, also known as CKAP-5, is a microtubule-associated protein that is involved in microtubule dynamics required for mitotic spindle formation. Specifically, chTOG protects kinetochore microtubules from depolymerization and plays an important role in centrosomal microtubule assembly. ch-TOG is typically overexpressed in tumor cells, may therefore be associated with cancer development. ch-TOG antibodies are useful tools for cytosleletal research and for some cancer studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKAP5