CETP Antibody

Western Blot: CETP Antibody [NBP3-11019] - Staining of HepG2 cell lysate (A) and Negative control Caco-2 cell lysate (B) (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

CETP Antibody Summary

• Description: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence GNAHACSKGTSHEA, from the N Terminus of the protein sequence according to NP_000069.2; NP_001273014.1.
• Epitope: GNAHACSKGTSHEA
• Specificity: This antibody is expected to recognise isoform s1 and 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CETP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx 48kDa band observed in lysates of cell line HepG2 (calculated MW of 47.9kDa according to NP_001273014.1. Primary incubation 1 hour at room temperature. Negative Control: CaCo-2 cell lysate.

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CETP Antibody

• BPIFF
• CETP
• cholesteryl ester transfer protein
• cholesteryl ester transfer protein, plasma
• HDLCQ10
• Lipid Transfer Protein I

Background

It has been proposed that high density lipoproteins (HDLs) function jointly with lecithin:cholesterol acyltransferase and CETP to facilitate cholesterol transport from tissues to the liver. CETP is responsible for neutral lipid transfer activity in plasma in numerous species. Since CETP is able to accelerate specifically the exchange of lipid components between pro- and anti-atherogenic lipoprotein fractions, it may be a key determinant of the global atherogenicity of the plasma lipoprotein profile and arises as a possible target in atherosclerosis prevention. CETP has an important role in reverse cholesterol transport and shaping and affecting the composition of plasma lipoproteins. In general elevated levels of CETP have been associated with increased risk of coronary heart disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CETP