Cerberus 1 Antibody (225807)

• Reactivity: Mu
• Applications: WB
• Clone: 225807
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cerberus 1 Antibody (225807) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
  Asp18-Pro272
  Accession # O55233
• Specificity: Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Cerberus, recombinant mouse (rm) DAN, or rmGremlin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CER1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cerberus 1 Antibody (225807)

• CER1
• cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily)
• Cerberus 1
• cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
• cerberus
• cerberus-related 1
• Cerberus-related protein
• DAN domain family member 4
• DAND4
• MGC119894
• MGC119895
• MGC96951

Background

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
3. Biben, C. et al. (1998) Dev. Biol. 194:135.
4. Piccolo, S. et al. (1999) Nature 397:707.
5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
6. Tavares, A.T. et al. (2007) Development 134:2051.
7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
8. Bouwmeester, T. et al. (1996) Nature 382:595.
9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
10. Glinka, A. et al. (1997) Nature 389:517.
11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cerberus 1 Antibody (MAB1986)(10)

Publications using MAB1986

• Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C; Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation Nature communications 2024-06-13 [PMID: 38871742] (Immunohistochemistry, Human)
• Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse)
• de Jong, MA;Adegeest, E;Bérenger-Currias, NMLP;Mircea, M;Merks, RMH;Semrau, S; The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion PLoS computational biology 2024-02-01 [PMID: 38306399] (Immunohistochemistry, Mouse)
• Kenzo Ivanovitch, Pablo Soro-Barrio, Probir Chakravarty, Rebecca A. Jones, Donald M. Bell, S. Neda Mousavy Gharavy et al. Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak PLOS Biology 2021-05-17 [PMID: 33999917]
• Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan et al. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro Developmental Cell 2021-02-08 [PMID: 33378662]
• C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G Basement membrane remodelling regulates mouse embryogenesis Nature, 2020-05-06;582(7811):253-258. 2020-05-06 [PMID: 32523119] (IHC, Mouse)
• Taroh Kinoshita A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO J. Cell Biol., 2016-11-23;215(5):705-718. 2016-11-23 [PMID: 27881714] (IHC, Mouse)
• Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells PLoS ONE 2013-05-22 [PMID: 23717584]
• Lijun Chi, Ulla Saarela, Antti Railo, Renata Prunskaite-Hyyryläinen, Ilya Skovorodkin, Shelagh Anthony et al. A Secreted BMP Antagonist, Cer1, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development PLoS ONE 2011-11-17 [PMID: 22114682]
• Torres-Padilla ME, Richardson L, Kolasinska P, Meilhac SM, Luetke-Eversloh MV, Zernicka-Goetz M The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation. Dev. Biol., 2007-07-03;309(1):97-112. 2007-07-03 [PMID: 17662710] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CER1
• Uniprot:
  • O55233()