Cerberus 1 Antibody (225807) [Alexa Fluor® 350]

• Applications: WB
• Clone: 225807
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

Cerberus 1 Antibody (225807) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
  Asp18-Pro272
  Accession # O55233
• Specificity: Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Cerberus, recombinant mouse (rm) DAN, or rmGremlin.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.