Ceramide Kinase Antibody [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Ceramide Kinase Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal portion of human Ceramide Kinase (between residues 50-150). [Swiss-Prot Q8TCT0]
Localization
Cytoplasmic and membraneous, cytoplasmic vesicles
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CERK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Ceramide Kinase antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is seen at ~65 kDa representing the Ceramide Kinase protein, and in Immunocytochemistry/Immunofluorescence vesicular cytoplasmic staining was observed in A431 cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen has 86% homology to mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ceramide Kinase Antibody [FITC]

  • ceramide kinase
  • dA59H18.2
  • DKFZp434E0211
  • EC 2.7.1.138
  • FLJ21430
  • FLJ23239
  • hCERKLipid kinase 4
  • KIAA1646dA59H18.3
  • lipid kinase LK4
  • LK4Acylsphingosine kinase
  • MGC131878

Background

Ceramide kinase (CERK) belongs to diacylglycerol kinase family of lipid kinases and it catalyzes specifically the phosphorylation of ceramide to generate ceramide 1-phosphate (C1P). CERK acts primarily on natural as well as analog ceramides such as C6, C8, C16 ceramides, and C8-dihydroceramide, to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids including sphingosines. Localized in the cytoplasm and membrane as peripheral membrane protein, CERK is the only enzyme known to produce C1P, a signaling molecule which shows positive effect on cell proliferation or survival and also regulates cytosolic phospholipase A2 (cPLA2) activity. CERK/C1P signaling is implicated in mast cell activation, eicosanoid signaling, apoptosis, cellular immune response/inflammation, emotional behavior, angiogenesis, phagolysosome formation etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CERK