Ceramide Kinase Antibody [DyLight 405]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 405

Ceramide Kinase Antibody [DyLight 405] Summary

• Immunogen: A synthetic peptide made to an internal portion of human Ceramide Kinase (between residues 50-150). [Swiss-Prot# Q8TCT0]
• Localization: Cytoplasmic and membraneous, cytoplasmic vesicles
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CERK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Ceramide Kinase Antibody [DyLight 405]

• Ceramide Kinase
• CERK
• dA59H18.2
• DKFZp434E0211
• EC 2.7.1.138
• FLJ21430
• FLJ23239
• hCERK
• hCERKLipid kinase 4
• KIAA1646
• KIAA1646dA59H18.3
• Lipid kinase 4
• lipid kinase LK4
• LK4
• LK4Acylsphingosine kinase
• MGC131878

Background

Ceramide kinase (CERK) belongs to diacylglycerol kinase family of lipid kinases and it catalyzes specifically the phosphorylation of ceramide to generate ceramide 1-phosphate (C1P). CERK acts primarily on natural as well as analog ceramides such as C6, C8, C16 ceramides, and C8-dihydroceramide, to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids including sphingosines. Localized in the cytoplasm and membrane as peripheral membrane protein, CERK is the only enzyme known to produce C1P, a signaling molecule which shows positive effect on cell proliferation or survival and also regulates cytosolic phospholipase A2 (cPLA2) activity . CERK/C1P signaling is implicated in mast cell activation, eicosanoid signaling, apoptosis, cellular immune response/inflammation, emotional behavior, angiogenesis, phagolysosome formation etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CERK