Ceramide Kinase Antibody [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

Ceramide Kinase Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to an internal portion of human Ceramide Kinase (between residues 50-150). [Swiss-Prot Q8TCT0]
Localization
Cytoplasmic and membraneous, cytoplasmic vesicles
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CERK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Ceramide Kinase antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is seen at ~65 kDa representing the Ceramide Kinase protein, and in Immunocytochemistry/Immunofluorescence vesicular cytoplasmic staining was observed in A431 cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen has 86% homology to mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ceramide Kinase Antibody [DyLight 488]

  • ceramide kinase
  • dA59H18.2
  • DKFZp434E0211
  • EC 2.7.1.138
  • FLJ21430
  • FLJ23239
  • hCERKLipid kinase 4
  • KIAA1646dA59H18.3
  • lipid kinase LK4
  • LK4Acylsphingosine kinase
  • MGC131878

Background

Ceramide kinase (CERK) is a novel lipid kinase. It is responsible for catalyzing the phosphorylation that produces a signaling lipid called ceramide-1-phosphate (C1P). C1P is an important biological mediator that induces inflammatory pathways such as those blocked by many allergy medications. Findings suggest that CERK could be a novel target for a new generation of anti-inflammatory/anti-trauma therapeutics dealing with such conditions as arthritis, asthma, multiple sclerosis, lung and colon cancers and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-2911B
DyLight 550 Labeled NB100-2911R
DyLight 650 Labeled NB100-2911C
HRP Labeled NB100-2911H

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Bioinformatics Tool for Ceramide Kinase Antibody (NB100-2911G)

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Bioinformatics

Gene Symbol CERK