CENPB Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369] - Detection of Human CENP-B by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B ...read more
Immunohistochemistry: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used ...read more
Immunocytochemistry/ Immunofluorescence: CENPB Antibody [NB100-2369] - Detection of Human CENP-B by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B ...read more
Immunohistochemistry: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CENPB Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 540 and the C-terminus (residue 599) of human Centromere Protein B using the numbering given in entry SwissProt entry P07199 (GeneID 1059).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CENPB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
Application Notes
Formaldehyde-Fixed Cells permeabilized with Triton-X 100 is recommended. Likely to work with frozen sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CENPB Antibody

  • CENP-B
  • centromere autoantigen B
  • centromere protein B (80kD)
  • Centromere protein B
  • centromere protein B, 80kDa
  • major centromere autoantigen B

Background

This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CENPB
Entrez
Uniprot