CEBP epsilon Antibody (695345)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP epsilon at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

CEBP epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 695345
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CEBP epsilon Antibody (695345) Summary

• Immunogen: E. coli-derived recombinant human CEBP epsilon
  Thr151-Arg210
  Accession # Q15744
• Specificity: Detects human CEBP epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEBP alpha, beta, delta, gamma, or zeta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEBPE
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEBP epsilon Antibody (695345)

• C/EBP-epsilon
• CCAAT/enhancer binding protein (C/EBP), epsilon
• CCAAT/enhancer-binding protein epsilon
• CEBP epsilon
• CEBPE
• CRP1C/EBP epsilon

Background

CCAAT-enhancer binding protein epsilon (CEBP epsilon ) is a 32 kDa basic-leucine zipper transcription factor that is expressed in myeloblasts, granulocytes, and eosinophils. Multiple isoforms of human CEBP epsilon are generated by alternate splicing and alternative translation initiation sites. All four isoforms contain the bZIP domain (aa 208-267) but exert different effects on myeloid differentiation. Full length CEBP epsilon and the 30 kDa isoform (aa 33-281) cooperate with c-Myb to activate the transcription of genes involved in myeloid differentiation. The 27 kDa isoform (which lacks aa 69-97) and the 14 kDa isoform (which begins at Met153) function as transcriptional repressors in eosinophils. Within aa 151-210, human CEBP epsilon shares 93% amino acid sequence identity with mouse and rat CEBP epsilon, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEBPE
• Uniprot:
  • Q15744()