CEBP alpha Antibody (692716) [Janelia Fluor® 646]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 692716
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

CEBP alpha Antibody (692716) [Janelia Fluor® 646] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human CEBP alpha
  Met1-Ala124
  Accession # P49715
• Specificity: Detects human CEBP alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEBPA
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB7094. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CEBP alpha Antibody (692716) [Janelia Fluor® 646]

• C/EBP alpha
• C/EBP-alpha
• CCAAT/enhancer binding protein (C/EBP), alpha
• CCAAT/enhancer-binding protein alpha
• CEBP alpha
• CEBP
• CEBPA

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEBPA