CEACAM5/CD66e Antibody (1C10)

Product Discontinued

CEACAM5/CD66e Antibody (1C10) Summary

• Immunogen: This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with human natural tumor-derived purified carcinoembryonic antigen.
• Epitope: Epitope Group 2
• Localization: Plasma membrane
• Specificity: Antibodies are not cross-reacting with human leukocytes in cytofluorometry.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: ELISA, Immunohistochemistry-P Antigen retrieval is not essential but may optimise staining. This conjugate antibody can be used for detection in sandwich ELISA with NB110-8364 (coating). Optimal dilutions and concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Tris (pH 7.5) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CEACAM5/CD66e Antibody (1C10)

• Carcinoembryonic antigen
• carcinoembryonic antigen-related cell adhesion molecule 5
• CD66e antigen
• CD66e
• CEA
• CEACAM5
• CEACAM-5
• CEACD66e
• DKFZp781M2392
• Meconium antigen 100

Background

Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM5
• Entrez:
  • 1048(Human)