CDK8 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

CDK8 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human CDK8
  Asn380-Tyr464
  Accession # P49336
• Specificity: Detects human CDK8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CDK2, rhCDK4, and CDK6 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDK8 Antibody [Alexa Fluor® 532]

• CDK8 protein kinase
• CDK8
• Cell division protein kinase 8
• cyclin-dependent kinase 8
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• K35
• Mediator complex subunit CDK8
• Mediator of RNA polymerase II transcription subunit CDK8
• MGC126074
• MGC126075
• Protein kinase K35

Background

CDK8 (cyclin-dependent kinase 8; also K35) is a 53-55 kDa member of the CDC2/CDKX subfamily, CMGC Ser/Thr protein kinase family of enzymes. Together with cyclin C, MED12 and MED13, CDK8 forms a "CDK8 module" that contributes to the structure of a four module Mediator complex. CDK8 would appear to have multiple functions. It can block transcription, and the re-initiation of transcription; it can also promote the transcription of thyroid hormone target genes; and it appears to phosphorylate the ICD of Notch, promoting its degradation. Human CDK8 is 464 amino acids (aa) in length. It contains one protein kinase domain (aa 21-335) and two utilized phosphorylation sites at Thr410 and Ser413. There are two potential alternative start sites at Met174 and Met61. Over aa 380-464, human CDK8 shares 98% aa identity with mouse CDK8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.