CDK8 Antibody [mFluor Violet 500 SE]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: mFluor Violet 500 SE

CDK8 Antibody [mFluor Violet 500 SE] Summary

• Immunogen: E. coli-derived recombinant human CDK8
  Asn380-Tyr464
  Accession # P49336
• Specificity: Detects human CDK8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CDK2, rhCDK4, and CDK6 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CDK8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CDK8 Antibody [mFluor Violet 500 SE]

• CDK8 protein kinase
• CDK8
• Cell division protein kinase 8
• cyclin-dependent kinase 8
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• K35
• Mediator complex subunit CDK8
• Mediator of RNA polymerase II transcription subunit CDK8
• MGC126074
• MGC126075
• Protein kinase K35

Background

Cdk8 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK8