Cdk7 Antibody

Western Blot: Cdk7 Antibody [NBP2-42500] - Analysis of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cdk7 Antibody Summary

• Description: Novus Biologicals Goat Cdk7 Antibody (NBP2-42500) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cdk7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HRSEAKDGINRT corresponding to internal region according to NP_001790.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in Jurkat lysates (calculated MW of 39.0 kDa band according to NP_001790.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cdk7 Antibody

• CAK1Xenopus laevis, cdk-activating kinase)
• Cdk7
• CDK-activating kinase
• CDKN7
• Cell division protein kinase 7
• cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase)
• cyclin-dependent kinase 7
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• EC 2.7.11.24
• MO15
• p39 Mo15
• serine/threonine kinase stk1,39 kDa protein kinase
• serine/threonine protein kinase 1
• serine/threonine protein kinase MO15
• STK1
• TFIIH basal transcription factor complex kinase subunit

Background

Cdk7 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cdk7 Antibody (NBP2-42500)(1)

Publication using NBP2-42500

• Bochar DA, Pan ZQ, Knights R et al. Inhibition of transcription by the trimeric cyclin-dependent kinase 7 complex. J Biol Chem 1999-05-01 [PMID: 10224071]

Bioinformatics

• Gene Symbol: CDK7
• Entrez:
  • 1022(Human)