| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5254-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26664-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58877-25ul | |||
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0010.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk4-Antibody-Immunohistochemistry-Paraffin-NBP1-31308-img0006.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0009.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024205643.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024209146.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-910202420104465.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0018.jpg "Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell")
![Immunohistochemistry: CDK4 Antibody [NBP2-32288] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunohistochemistry-NBP2-32288-img0017.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary, Antibody Dilution: 1 : 2000 Secondary Antibody: Anti-rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CDK4.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0016.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Western blot analysis of HeLa (A) and K562 (B) cell lysate using Cdk4 antibody (NBP2-32288) at 2ug/ml.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0019.jpg)
![Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Western-Blot-NBP3-15344-img0008.jpg "Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunofluorescence analysis of HeLa cells using CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunocytochemistry-Immunofluorescence-NBP3-15344-img0007.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0005.jpg)
![Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517162385.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517284930.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-72202512435398.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245317.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245373.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245387.jpg)
![Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Western-Blot-NBP2-88912-img0007.jpg "Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunocytochemistry-Immunofluorescence-NBP2-88912-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human endometrium shows strong nuclear positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human liver cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0005.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0012.jpg "Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Immunocytochemistry-Immunofluorescence-H00001019-M03-img0009.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0001.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0002.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0007.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0010.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0011.jpg)
![ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-ELISA-H00001019-M03-img0008.jpg)
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0009.jpg "Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00001019-M04-img0006.jpg)
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0007.jpg)
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0008.jpg)
![ELISA: CDK4 Antibody (2G7) [H00001019-M04] - Detection limit for recombinant GST tagged CDK4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-ELISA-H00001019-M04-img0005.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0010.jpg "Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Immunocytochemistry-Immunofluorescence-H00001019-M09-img0007.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0008.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0009.jpg)
![ELISA: CDK4 Antibody (8A10) [H00001019-M09] - Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-ELISA-H00001019-M09-img0005.jpg)
![Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26664_rabbit-cdk4-mab-8f2-262202413221518.jpg "Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Western Blot: CDK4 Antibody (8F2) [NBP3-26664] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: CDK4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 21 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26664_rabbit-cdk4-mab-8f2-28220241140923.jpg)
![Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32175_mouse-cdk4-mab-5-c3-g3-r-2782024163165.jpg "Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP2-58877] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli, nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58877-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP2-58877] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli, nuclear membrane & cytosol. Antibody staining is shown in green.")