CDK4 Antibody (8H4) - BSA Free

Western Blot: CDK4 Antibody (8H4) [NBP3-26665] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate. All lanes: CDK4 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 21 kDa Observed band size: 34 kDa

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 8H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDK4 Antibody (8H4) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human CDK4 [UniProt P11802]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDK4 Antibody (8H4) - BSA Free

• CDK4
• Cell division protein kinase 4
• CMM3
• cyclin-dependent kinase 4
• EC 2.7.11
• EC 2.7.11.22
• melanoma cutaneous malignant, 3
• MGC14458
• PSK-J3
• PSK-J3cell division kinase 4

Background

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK4