CDC7 Antibody (SY21-01)

Western Blot: CDC7 Antibody (SY21-01) [NBP2-67421] - Analysis of CDC7 Kinase on different lysates using anti-CDC7 antibody at 1:1000 dilution. Lane 1 (positive control): Jurkat cells lysate. Lane 2: HeLa cell lysate.

Flow Cytometry: CDC7 Antibody (SY21-01) [NBP2-67421] - Analysis of A431 cells with CDC7 Kinase antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Discontinued

CDC7 Antibody (SY21-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CDC7 aa 1-50 / 574. (SwissProt: O00311 Human; SwissProt: Q9Z0H0 Mouse; Unigene: 20842 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC7 Antibody (SY21-01)

• CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1
• CDC7 cell division cycle 7 (S. cerevisiae)
• CDC7
• CDC7L1
• CDC7L1MGC117361
• CDC7-related kinase
• cell division cycle 7 (S. cerevisiae)
• cell division cycle 7 homolog (S. cerevisiae)
• cell division cycle 7-like protein 1
• cell division cycle 7-related protein kinase
• EC 2.7.11.1
• HsCdc7
• Hsk1
• huCdc7
• MGC126237
• MGC126238

Background

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CDC7 Antibody (NBP2-67421) (1)

Average Rating: 2

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 08/27/2020

Summary

• Application: Simple Western
• Sample Tested: COLO-205 Cell Line,HCT116 cell line lysate
• Species: Human
• Lot: HJ1017

Bioinformatics

• Gene Symbol: CDC7