CDC5L Overexpression Lysate (Native)

Images

 
Western Blot: CDC5L Overexpression Lysate (Adult Normal) [NBL1-09013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC5L.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CDC5L Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201702 and based on accession number NM_001253. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CDC5 cell division cycle 5-like (S. pombe) (CDC5L), mRNA.
Gene
CDC5L

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
92.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CDC5L

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDC5L Overexpression Lysate (Native)

  • CDC5 (cell division cycle 5, S. pombe, homolog)-like
  • CDC5 cell division cycle 5-like (S. pombe)
  • CDC5
  • Cdc5-like protein
  • Cdc5-related protein
  • CEF1
  • cell division cycle 5-like protein
  • dJ319D22.1 (CDC5-like protein)
  • dJ319D22.1
  • hCDC5
  • KIAA0432
  • PCDC5RPCDC5-LIKE
  • Pombe cdc5-related protein

Background

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CDC5L Lysate (NBL1-09013) (0)

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Bioinformatics

Gene Symbol CDC5L