CDC5L Antibody (JJ091-05)

Western Blot: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CDC5L antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC5L Antibody (JJ091-05) [NBP2-67901] - Staining CDC5L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of paraffin-embedded human kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of paraffin-embedded mouse kidney tissue using anti-CDC5L antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDC5L Antibody (JJ091-05) [NBP2-67901] - Analysis of paraffin-embedded mouse lung tissue using anti-CDC5L antibody. Counter stained with hematoxylin.

Product Discontinued

CDC5L Antibody (JJ091-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human CDC5L aa 320-370. (SwissProt: Q99459 Human; SwissProt: Q6A068 Mouse; SwissProt:O08837 Rat)
• Localization: Cytoplasm, Nucleus, Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC5L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC5L Antibody (JJ091-05)

• CDC5 (cell division cycle 5, S. pombe, homolog)-like
• CDC5 cell division cycle 5-like (S. pombe)
• CDC5
• Cdc5-like protein
• Cdc5-related protein
• CEF1
• cell division cycle 5-like protein
• dJ319D22.1 (CDC5-like protein)
• dJ319D22.1
• hCDC5
• KIAA0432
• PCDC5RPCDC5-LIKE
• Pombe cdc5-related protein

Background

CDC5L is encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC5L