CDC40 Antibody (SR3222) - BSA Free

Western Blot: CDC40 Antibody (SR3222) [NBP3-27760] - Western blot analysis of CDC40 expression in (1) HeLa cell lysate; (2) K562 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SR3222
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDC40 Antibody (SR3222) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human CDC40 (Uniprot #: O60508)
• Specificity: Detects endogenous levels of total CDC40
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC40
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDC40 Antibody (SR3222) - BSA Free

• cell division cycle 40 homolog (S. cerevisiae)
• cell division cycle 40 homolog (yeast)
• Cell division cycle 40 homolog
• Ehb3
• EH-binding protein 3
• hPRP17
• MGC102802
• pre-mRNA splicing factor 17
• pre-mRNA-processing factor 17
• PRP17 homolog
• PRP17EHB3PRPF17FLJ10564

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC40