Cdc23 Antibody

Western Blot: Cdc23 Antibody [NB100-59829] - Detection of human and mouse CDC23/APC8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Western Blot: Cdc23 Antibody [NB100-59829] - Detection of Human and Mouse CDC23/APC8 on HeLa whole cell lysate using NB100-59829. CDC23/APC8 was also immunoprecipitated by rabbit anti-CDC23/APC8 antibody NB100-59830.

Immunoprecipitation: Cdc23 Antibody [NB100-59829] - Detection of human CDC23/APC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC23/APC8 antibody NB100-59829 used for IP at 3 ug per reaction. CDC23/APC8 was also immunoprecipitated by another rabbit anti-CDC23/APC8 antibody. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cdc23 Antibody Summary

• Description: Novus Biologicals Rabbit Cdc23 Antibody (NB100-59829) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human cell division cycle 23 (anaphase-promoting complex subunit 8) using the numbering given in entry NP_004652.2 (GeneID 8697).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC23
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cdc23 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cdc23 Antibody

• ANAPC8anaphase promoting complex subunit 8
• Anaphase-promoting complex subunit 8
• APC8CDC23 (cell division cycle 23, yeast, homolog)
• cell division cycle 23 homolog (S. cerevisiae)
• cell division cycle protein 23 homolog
• CUT23
• Cyclosome subunit 8

Background

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC23
• Entrez:
  • 8697(Human)
• Uniprot:
  • Q9UJX2(Mouse)