Apc1 Antibody

Images

 
Western Blot: Apc1 Antibody [NBP1-77375] - analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: Apc1 Antibody [NBP1-77375] - Human brain tissue with APC1 antibody at 5 ug/mL.
Flow Cytometry: Apc1 Antibody [NBP1-77375] - APC1 expression in human lymphocytes. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Apc1 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1. The immunogen is located within amino acids 1810 - 1860 of APC1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANAPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Flow data from customer review.
Control Peptide
Apc1 Peptide (NBP1-77375PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77375 in the following applications:

Publications
Read Publication using NBP1-77375.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apc1 Antibody

  • anaphase promoting complex subunit 1
  • anaphase-promoting complex 1 (meiotic checkpoint regulator)
  • anaphase-promoting complex subunit 1
  • APC1Testis-specific gene 24 protein
  • Cyclosome subunit 1
  • Mitotic checkpoint regulator
  • TSG24MCPR

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apc1 Antibody (NBP1-77375)(1)

We have publications tested in 1 application: FLOW.


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Review for Apc1 Antibody (NBP1-77375) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-77375:
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Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry Apc1 NBP1-77375
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reviewed by:
Anonymous
Flow Human 06/30/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Primary Anitbody

Dilution Ratio1:100 in BD Perm/Wash Buffer

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Apc1 Antibody (NBP1-77375) (0)

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Secondary Antibodies

 

Isotype Controls

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Apc1 NBP1-77375

Bioinformatics Tool for Apc1 Antibody (NBP1-77375)

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Research Areas for Apc1 Antibody (NBP1-77375)

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Blogs on Apc1

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Recent Reviews

5
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1
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Anonymous
06/30/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol ANAPC1
Uniprot