Cdc23 Overexpression Lysate (Native)

Images

 
Western Blot: Cdc23 Overexpression Lysate (Adult Normal) [NBL1-08993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdc23.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cdc23 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC224973 and based on accession number NM_004661. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cell division cycle 23 homolog (S. cerevisiae) (CDC23), mRNA.
Gene
CDC23

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cdc23

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cdc23 Overexpression Lysate (Native)

  • ANAPC8anaphase promoting complex subunit 8
  • Anaphase-promoting complex subunit 8
  • APC8CDC23 (cell division cycle 23, yeast, homolog)
  • cell division cycle 23 homolog (S. cerevisiae)
  • cell division cycle protein 23 homolog
  • CUT23
  • Cyclosome subunit 8

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cdc23 Lysate (NBL1-08993) (0)

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Bioinformatics

Gene Symbol CDC23