CDC2/CDK1 [p Tyr15] Antibody

Product Discontinued

CDC2/CDK1 [p Tyr15] Antibody Summary

• Immunogen: Synthetic peptide (Human) conjugated to KLH.
• Epitope: anti-Cdc 2 antibody, anti-CDK 1 antibody, anti-CDK1 antibody, anti-Cell division control protein 2 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell division cycle 2 G1 to S and G2 to M antibody, anti-Cell Divsion Cycle 2 Protein
• Modification: p Tyr15
• Localization: Nuclear
• Specificity: Anti-phospho-cdc2 (phosphotyrosine 15) detects cdc2 and cdk2 only when catalytically inactivated by phosphorylation at Tyr15. It does not react with non-phosphorylated cdc2, nor cdk4, cdk6 or cdk7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: Use at a dilution of 1/1000. Predicted molecular weight: 34 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  CDC2/CDK1 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Mouse.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES, 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for CDC2/CDK1 [p Tyr15] Antibody

• CDC2
• CDC28A
• CDC2MGC111195
• CDK1
• cell cycle controller CDC2
• Cell division control protein 2 homolog
• Cell division protein kinase 1
• cyclin-dependent kinase 1
• DKFZp686L20222
• EC 2.7.11.22
• EC 2.7.11.23
• G1 to S and G2 to M
• p34 protein kinase
• P34CDC2

Background

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK1
• Entrez:
  • 983(Human)
  • 12534(Mouse)