CDC2/CDK1 Antibody (SM01-44)

Western Blot: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Analysis of CDK1 on Jurkat cells lysates using anti-CDK1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Staining CDK1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Analysis of paraffin-embedded human tonsil tissue using anti-CDK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Analysis of Jurkat cells with CDK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Staining CDK1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Staining CDK1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (SM01-44) [NBP2-66764] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CDK1 antibody. Counter stained with hematoxylin.

Product Discontinued

CDC2/CDK1 Antibody (SM01-44) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Full length protein
• Localization: Nucleus, Cytoplasm, Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 1%BSA, 40%Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC2/CDK1 Antibody (SM01-44)

• CDC2
• CDC28A
• CDC2MGC111195
• CDK1
• cell cycle controller CDC2
• Cell division control protein 2 homolog
• Cell division protein kinase 1
• cyclin-dependent kinase 1
• DKFZp686L20222
• EC 2.7.11.22
• EC 2.7.11.23
• G1 to S and G2 to M
• p34 protein kinase
• P34CDC2

Background

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK1