CDC16 Antibody

Images

 
Western Blot: CDC16 Antibody [NBP1-77155] - Human liver tissue lysate with CDC16 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: CDC16 Antibody [NBP1-77155] - Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: CDC16 Antibody [NBP1-77155] - Human liver tissue with CDC16 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CDC16 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6. The immunogen is located within the first 50 amino acids of APC6.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC16
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Positive Control
Brain Cerebral Peduncles Lysate (NB820-59179)
Control Peptide
CDC16 Peptide (NBP1-77155PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC16 Antibody

  • ANAPC6subunit 6
  • CDC16 (cell division cycle 16, S. cerevisiae, homolog)
  • CDC16 cell division cycle 16 homolog (S. cerevisiae)
  • CDC16 homolog
  • CDC16Hs
  • cell division cycle 16 homolog (S. cerevisiae)
  • cell division cycle protein 16 homolog
  • Cyclosome subunit 6

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDC16 Antibody (NBP1-77155) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDC16
Uniprot