CD84/SLAMF5 Antibody (6) - Azide and BSA Free

Western Blot: CD84/SLAMF5 Antibody (6) [NBP2-89267] - Lane A: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDa Observed band size:39 kDa

Immunoprecipitation: CD84/SLAMF5 Antibody (6) [NBP2-89267] - Lane A:0.5 mg Raw264.7 Whole Cell Lysate 0.5 uL anti-CD84 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD84 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 62 kDaObserved band size: 62 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD84/SLAMF5 Antibody (6) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD84/SLAMF5 extracellular domain (Met 1-Gly 225; Accession#: NP_003865.1).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD84
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CD84/SLAMF5 Antibody (6) - Azide and BSA Free

• CD84 antigen (leukocyte antigen)
• CD84 antigen
• CD84 molecule
• CD84
• Cell surface antigen MAX.3
• DKFZp781E2378
• hCD84
• hly9-beta
• leucocyte differentiation antigen CD84
• leukocyte antigen CD84
• Leukocyte differentiation antigen CD84
• Ly-9B
• mCD84
• Signaling lymphocytic activation molecule 5
• SLAM family member 5
• SLAMF5
• SLAMF5LY9B

Background

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD84