CD8 beta Antibody (H35-17.2) - BSA Free

Flow Cytometry: CD8 beta Antibody (H35-17.2) [NBP1-43449] - Analysis using the FITC conjugate of NBP1-43449. Staining of BALB/c splenocytes with Anti-Mouse CD8a APC and 0.125 ug of Rat IgG2b k Isotype Control FITC (left) or 0.125 ug of Anti-Mouse CD8b FITC (right). Cells in the lymphocyte gate were used for analysis,

Flow Cytometry: CD8 beta Antibody (H35-17.2) [NBP1-43449] - Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD8b Purified (filled histogram) followed by Anti-Rat IgG FITC. Cells in the lymphocyte gate were used for analysis.

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: H35-17.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD8 beta Antibody (H35-17.2) - BSA Free Summary

• Description: Novus Biologicals Rat CD8 beta Antibody (H35-17.2) - BSA Free (NBP1-43449) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CD8.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD8B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 ug/10^6 cells in 100 ul volume
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD8 beta Antibody (H35-17.2) - BSA Free

• CD8 antigen, beta polypeptide 1 (p37)
• CD8 beta
• CD8b antigen
• CD8b molecule
• CD8b
• CD8B1
• CD8B1P37
• Leu2
• LY3
• LYT3
• MGC119115
• P37
• T lymphocyte surface glycoprotein beta chain
• T-cell surface glycoprotein CD8 beta chain

Background

The H35-17.2 monoclonal antibody reacts with the mouse CD8beta molecule. The CD8beta chain associates with the CD8alpha chain to form the CD8alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alphabeta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD8 beta Antibody (NBP1-43449). (Showing 1 - 1 of 1 FAQ).

1. May we ask the suggested dilution titers while using NB200-578 and NBP1-43449 for IHC?
   • A good starting point for optimization in IHC for both products would be 1:100.

Bioinformatics

• Gene Symbol: CD8B
• Entrez:
  • 12526(Mouse)
• Uniprot:
  • P10300(Mouse)