CD8 beta Antibody (BU88)

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, ICC/IF
• Clone: BU88
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD8 beta Antibody (BU88) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11543)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human CD8 beta (Uniprot: P10966)
• Localization: Cell surface
• Marker: Cytotoxic / Suppressor T-Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD8B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD8 beta Antibody (BU88)

• CD8 antigen, beta polypeptide 1 (p37)
• CD8 beta
• CD8b antigen
• CD8b molecule
• CD8b
• CD8B1
• CD8B1P37
• Leu2
• LY3
• LYT3
• MGC119115
• P37
• T lymphocyte surface glycoprotein beta chain
• T-cell surface glycoprotein CD8 beta chain

Background

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8 beta. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8 alpha chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD8 beta Antibody (NBP2-44753)(2)

Publications using NBP2-44753

• Qu F, Brough SC, Michno W et al. Crosstalk between small-cell lung cancer cells and astrocytes mimics brain development to promote brain metastasis Nature cell biology 2023-10-01 [PMID: 37783795]
• Bao C, Wang F, Wang X et al. ThPOK and Runx3 Regulate the Differentiation of CD4+CD8??+ IELs in Oral Lichen Planus Research Square 2023-10-10 (IHC-P, ICC/IF, Human)
  
  Details:
  Dilution 1:500

Bioinformatics

• Gene Symbol: CD8B
• Uniprot:
  • P10966(Human)