CD57 Antibody (1088014) [Unconjugated]

PBMC were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CD57 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD57 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD57 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD57 Monoclonal Antibody (Catalog # MAB11708) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: 1088014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD57 Antibody (1088014) [Unconjugated] Summary

• Immunogen: Whole Cell
  Accession # Q9P2W7
• Specificity: Detects the CD57 carbohydrate epitope in Human PBMCs
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD57 Antibody (1088014) [Unconjugated]

• CD57
• HNK1
• HNK-1
• Human Natural Killer-1
• L2
• LEU7
• LEU-7

Background

CD57 is a glycoprotein with a molecular weight of approximately 100 kDa, classified within the group of adhesion molecules. It plays a significant role in immune regulation and cellular interactions, particularly influencing the functioning of natural killer (NK) cells and certain T-cells. CD57 is heavily involved in the biological processes of cytotoxic activity and immune response modulation. Altered levels of CD57 expression have been associated with several pathological conditions, including chronic infections, autoimmune diseases, and various malignancies. Crucially, CD57 serves as a key biomarker for immune senescence and the monitoring of NK cell and T-cell activities in diseases such as HIV and cancer.

1. Kared H, Martelli S, Ng TP, Pender SL, Larbi A. CD57 in human natural killer cells and T-lymphocytes. Cancer Immunol Immunother. 2016 Apr;65(4):441-52. doi: 10.1007/s00262-016-1803-z. Epub 2016 Feb 5. PMID: 26850637; PMCID: PMC11029668. 

2. Elias Junior E, Gubert VT, Bonin-Jacob CM, Puga MAM, Gouveia CG, Sichinel AH, Tozetti IA. CD57 T cells associated with immunosenescence in adults living with HIV or AIDS. Immunology. 2024 Jan;171(1):146-153. doi: 10.1111/imm.13707. Epub 2023 Oct 25. PMID: 37880915. 

3. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020 Nov 10;13(1):151. doi: 10.1186/s13045-020-00986-z. Erratum in: J Hematol Oncol. 2025 Mar 5;18(1):25. doi: 10.1186/s13045-025-01682-6. PMID: 33168037; PMCID: PMC7653700.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9P2W7()