CD53 Antibody (425514) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 Monoclonal Antibody (Catalog # MAB4624, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 425514
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD53 Antibody (425514) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CD53
  Met1-Leu219
  Accession # P19397
• Specificity: Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD53
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD53 Antibody (425514) [Unconjugated]

• antigen MOX44 identified by monoclonal MRC-OX44
• CD53 antigentetraspanin-25
• CD53 glycoprotein
• CD53 molecule
• CD53 tetraspan antigen
• CD53
• cell surface antigen
• Cell surface glycoprotein CD53
• MOX44
• MOX44transmembrane glycoprotein
• Tetraspanin-25
• TSPAN25
• Tspan-25
• TSPAN25leukocyte surface antigen CD53

Background

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein in the tetraspanin superfamily. CD53 is widely expressed on hematopoietic cells. Ligation of CD53 promotes cell activation and survival as well as homotypic cell‑cell adhesion. CD53 associates with other tetraspanins, MHC class I and II molecules, and integrin alpha 4 beta 1. Human CD53 shares 81%‑83% aa sequence identity with mouse and rat CD53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD53
• Uniprot:
  • P19397()