CD53 Antibody (161-2) [Allophycocyanin]

• Reactivity: Hu, Pm(-), Eq(-)
• Applications: Flow, ICC/IF, CyTOF-ready, ICC/IF
• Clone: 161-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CD53 Antibody (161-2) [Allophycocyanin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Clone 161-2 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human leukocytes
• Localization: Cell Surface
• Specificity: Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This monoclonal antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD53
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-47859. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Does not react with Baboon or Equine.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CD53 Antibody (161-2) [Allophycocyanin]

• antigen MOX44 identified by monoclonal MRC-OX44
• CD53 antigentetraspanin-25
• CD53 glycoprotein
• CD53 molecule
• CD53 tetraspan antigen
• CD53
• cell surface antigen
• Cell surface glycoprotein CD53
• MOX44
• MOX44transmembrane glycoprotein
• Tetraspanin-25
• TSPAN25
• tspan-25
• TSPAN25leukocyte surface antigen CD53

Background

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD53