CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).

Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

• Reactivity: Hu, Pm-Cm, RM
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, CyTOF-ready, Dual ISH-IHC
• Clone: Campath-1H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free (NBP2-52645) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow, Dual RNAscope ISH-IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human lymphocytes.
• Specificity: Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD52
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In vivo assay
  • Western Blot
• Application Notes: This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

• biosimilar antibody
• biosimilars
• Cambridge pathology 1 antigen
• CAMPATH-1 antigen
• CAMPATH-1
• CD52 antigen (CAMPATH-1 antigen)
• CD52 antigen
• CD52 molecule
• CD52
• CDW52 antigen (CAMPATH-1 antigen)
• CDw52
• Epididymal secretory protein E5
• He5
• HEL-S-171mP
• Human epididymis-specific protein 5

Background

Human CD52 is a glycosylphosphatidylinositol (GPI)anchored antigen expressed in a number of different tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD52