CD5 Antibody Pair [HRP]

Sandwich ELISA: CD5 Antibody Pair [HRP] [NBP2-79576] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP
• Standard Curve Range: 12.5-800 pg/ml (example only; lot dependent)
• Sensitivity: 12.5 pg/ml (example only; lot dependent)

CD5 Antibody Pair [HRP] Summary

• Description: Solid Phase sandwich ELISA for the quantitative determination of Mouse CD5.
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse CD5.
• Standard Curve Range: 12.5-800 pg/ml (example only; lot dependent)
• Sensitivity: 12.5 pg/ml (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: CD5

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for CD5 Antibody Pair [HRP]

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD5